Millard and Muriel Jacobs Genetics and Genomics Laboratory operates Illumina HiSeq2500 high throughput sequencer that features two run modes, rapid run and high output run mode, and has the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications including ChIP-Seq, RNA-Seq, small RNA analysis, de novo genome sequencing, mutation discovery, etc. and is easlily adaptable to different study sizes. Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, while the high output mode is well-suited for larger studies with more samples or when the greatest depth of coverage is required.
We'd be happy to talk to you talk to you about any aspect of high throughput sequencing from general technology overview to more specific questions related to project planning including currently supported applications, sequencing type and depth appropriate for a particular study, experimental design, etc. Please do not hesitate to contact us to schedule a meeting.
For instructions on how to access the data, description of file formats and some additional HTS-related documentation, visit these wiki pages.
Additional information and reading
Standard Illumina protocols used in the lab are listed here.